Table 1. Parameters of thermal denaturation and aggregation of bovine serum albumin ([BSA] = 1 mg/ml, 0.1 M Na-phosphate buffer, pH 7.0).

T, °C	$t_{0.5}^{\text{den}}$, min	γnon-agg,lim	γUhr	γUlr,agg	Rh,1, nm	Rh,2, nm	I2, counts/s
60	19±1	0.51±0.01	γUhr + γUlr,agg = 0.49±0.01		–	11.1±0.1	(3.2±0.1)∙103
65	13.6±0.6	0.35±0.01	0.51±0.03	0.14±0.03	10.3±0.3	12.8±0.3	(5.1±0.1)∙103
70	1.6±0.1	0.05±0.01	γUhr + γUlr,agg = 0.95±0.01		10.4±0.4	15.1±0.4	(12.1±0.1)∙103
80	1.5±0.1	0.06±0.01	γUhr + γUlr,agg = 0.94±0.01		–	–	–

Designations: $t_{0.5}^{\text{den}}$ is the time of half-conversion for the kinetic curves of BSA denaturation, γ_non-agg,lim is the portion of the unfolded protein that remains unincorporated in the large-sized aggregates for a long time (this species corresponds to A_st in Fig 6), γ_Uhr is the portion of the highly reactive unfolded form, γ_Ulr,agg is the portion of the low reactive unfolded form that is involved in the aggregation process by the attachment to the primary aggregates, I₂ is the value of the light scattering intensity reached after completion of the formation of the secondary aggregates, R_h,1 and R_h,2 are the hydrodynamic radius of the primary and the secondary aggregates, respectively.