Skip to main content
. 2016 Apr 21;11(4):e0154041. doi: 10.1371/journal.pone.0154041

Fig 8. GUS transient assays in tobacco leaves.

Fig 8

(A) The plasmids used in the transient assay. The CaMV 35S represents the full-length 35S promoter; p-mini35S represents the truncated 35S (–46 to +10 bp) promoter. The test construct consisted of the p-71bp-mini35S, in which the 71-bp region (–219 to –148 bp) identified in the ZmGAPP promoter was fused to the p-mini35S promoter to drive the GUS expression. (B) GUS activity in the transiently transformed tobacco leaves with constructs p-mini35S and p-71bp-mini35S under both normal and 200 mM NaCl or 18% (w/v) PEG 6000 treatment for 24 h. Results are mean ± SD from three experiments (n = 15). Different lowercase letters above the bars indicate significant differences at P < 0.05.