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. 2016 Apr 21;11(4):e0154229. doi: 10.1371/journal.pone.0154229

Fig 5. Concentration dependent stimulation of hGLP-1R internalisation by GLP-1 in the presence of antagonists Ex(9–39) and JANT-4.

Fig 5

HEK293 cells expressing the hGLP-1R were stimulated with GLP-1 at the indicated concentration for 60min in the presence of 100nM Ex(9–39) (left panel) or JANT-4 (right panel) and the internalisation of hGLP-1R was assessed by ELISA (A) and immunofluorescence (C) using the anti-GLP-1R antibody. (B) The EC50 of GLP-1 in the presence or absence of Ex(9–39) or JANT-4 for hGLP-1R internalisation. In immunofluorescence, GFP-tag of hGLP-1R (green) and the anti-GLP-1R antibody staining (red) overlay is shown in yellow and nuclear staining with DAPI in blue. Data are mean + SEM, n = 3, ** p<0.01; *** p <0.001.