Fig 1. Low mass Hep stimulates HARE-mediated NF-κB activated gene expression less effectively than UFH.

Cells expressing EV (white bars) or WT (black bars) HARE were grown and transfected with LUC recorder gene plasmids as described in Methods. The cells were incubated at 37°C for 4 h with 100 nM UFH or increasing concentrations of LMWH, as indicated, and then processed and analyzed for relative LUC activities. Values are the means ± SEM (n = 9) of the ratio of the two LUC activities determined from three independent experiments, with the value for unstimulated cells set as 1.0. Values for p compared WT cells with EV cells for each condition (*, p < 0.05; **, p 0.005) using ANOVA.