FIGURE 4.

Frequency of CD33⁺CD11b⁺ cells, monocytic MDSCs, granulocytic MDSCs, CD33⁺CD11b⁺HLA-DR⁺ cells, and Tregs in peripheral blood of healthy controls and HIV infected individuals. (A, B) Gating strategy of M-MDSCs and G-MDSCs by flow cytometric analysis. CD11b⁺CD33⁺/high cells were first selected from live PBMCs (A), and the CD14⁺/HLA-DR⁻/low population as M-MDSC (B). (C, D, F) Comparison of M-MDSCs, CD33⁺CD11b⁺HLA-DR⁺ cells, and Tregs between HIV-infected ART-naive individuals, healthy controls, and HIV-infected individuals on ART. ^∗P < 0.05: statistically significant difference from controls. (E) Correlation between CD33⁺CD11b⁺ cells frequencies and signal intensity of 12 autoantibody titers by microarray (NFI) (r² = 0.2703, P = 0.0404; linear regression). ART = antiretroviral therapy, HIV = human immunodeficiency virus, MDSC = myeloid-derived suppressor cell, NFI = net fluorescent intensity.