Fig. 1.

Inflammatory treatment increases pool size of DRG neurons displaying low-voltage activated (LVA) Ca²⁺ currents. (A) Cocktail of inflammatory mediators (100 nM BK, 2 μM ATP, 500 nM NE, 500 nM PGE2) increased percentage of LVA-positive neurons. Shown are exemplary current traces from control (top) and inflammatory cocktail-treated small-diameter DRG neurons recorded with whole-cell patch clamp using voltage protocol depicted above. Pie-charts summarize the percentage of LVA-positive neurons. Bar chart on the right summarizes the LVA current amplitudes in LVA-positive neurons in both conditions. (B) Exemplary micrographs of live cell imaging at 0 h (left) and 24 h (right) with or without inflammatory treatment (see Methods for detail). Bar chart on the right summarizes the percentage of morphologically intact neurons at the end of 24 h incubation in either condition. For (A) and (B) number of neurons is indicated within the charts; data from at least three independent preparations.