Fig. 4.
Expression of CaV3.2 T-type channel subunit in DRG neurons is not affected by the inflammatory treatment. (A) Pie-charts of the proportion of LVA-positive neurons in cultures incubated for 24 h with (right) or without (left) 5 μM MG132. Control group represents pooled data from all the control cultures used in this study. (B) Summary of mRNA levels in DRG cultures incubated for 24 h with either a vehicle control or the cocktail of inflammatory mediators (100 nM BK, 2 μM ATP, 500 nM NE, 500 nM PGE2) or each of these mediators individually at the specified concentration. (C) Exemplary confocal images of DRG neurons treated in the same way as in (B) (scale bar is 50 μM) and stained with anti-CaV3.2 antibody. (D) Summary of CaV3.2 staining intensity for conditions shown in (C). For (A–D) number of neurons is indicated within the charts; data from at least three independent preparations.