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. 2016 Mar 13;31(5):938–946. doi: 10.1093/humrep/dew030

Figure 2.

Figure 2

Automated 96-well high throughput comet assay (HT-COMET). (A) Each well was filled with a mix of low melting point agarose and either untreated or DNAse-treated (100 KU/ml) spermatozoa from patient #1 in eight groups of six wells. Median values of percentage DNA in the tail (B) and TEM (C) per well per group after an alkaline comet assay (n = 5) are shown. Median values of percentage DNA in the tail (D) and TEM (E) per well per group after a neutral comet assay (n = 3) are shown. No significant difference between groups was detected using the non-parametric Kruskal–Wallis test.