Table 1.
Media matrix used for the present study
| Media | Basal Medium | Carbon Source (%) | Auxin (mg L−1) | Cytokinin | Proline | Yeast Extract | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Maltose | Mannitol | Sucrose | Sorbitol | 2,4-D | NAA | BAP | KIN | (mg L−1) | (mg L−1) | ||
| RCM1 | MS | 5.00 | - | - | - | 3.00 | - | - | - | - | - |
| RCM2 | MS | 4.00 | - | - | - | 2.50 | - | 0.25 | 0.25 | 2.87 | - |
| RCM3 | MS | 2.00 | 1.00 | - | - | 2.00 | 0.50 | 0.50 | - | 2.87 | 500.00 |
| RCM4 | N6 | 5.00 | - | - | - | 3.00 | - | - | - | - | - |
| RCM5 | N6 | 4.00 | - | - | - | 2.50 | 0.25 | 0.25 | 2.87 | - | |
| RCM6 | N6 | 2.00 | 1.00 | - | - | 2.00 | 0.50 | 0.50 | 2.87 | 500.00 | |
| RR1 | MS | 3.00 | - | - | - | 1.00 | 2.50 | - | - | ||
| RR2 | MS | - | - | 2.00 | 1.00 | - | - | 0.10 | 2.50 | - | - |
| RR3 | N6 | 3.00 | - | - | - | - | - | 1.00 | 2.50 | - | - |
| RR4 | N6 | - | - | 2.00 | 1.00 | - | - | 0.10 | 2.50 | - | - |
| RSM | MS | - | - | 3.00 | - | - | - | 4.00 | 2.00 | - | - |
All RCM medium were supplemented with 0.05 % Casein hydrolase, 0.01 % Myo-inositol and 0.5 % Gelrite. Whereas RR medium contain only 0.01 % Myo-inositol and 0.5 % Gelrite