Table 2.
Transformation efficiency mediated by biolistic transformation in S. dulcis L
| Sl. Number of experimenta | Number of bombarded leaf explants | Number of responding leaf explants with kanamycin on selection | Number of Transgenic shoots/explant | Number of putative tranformants | Transformation efficiency (%)c | |
|---|---|---|---|---|---|---|
| Kanamycin resistant | GUS positiveb | |||||
| 1 | 25 | 12 | 4.1 ± 0.20 | 49 | 42 | 48.0 a |
| 2 | 25 | 15 | 3.3 ± 0.30 | 49 | 43 | 60.0 b |
| 3 | 25 | 12 | 3.7 ± 0.22 | 44 | 40 | 48.0 a |
| Mean | 25 | 14 | 3.7 ± 0.24 | 47 | 41 | 52 |
aPlants grown on RMBI-K medium
bFully mature putative transformants showing GUS expression
cMean values with significant difference in transformation effeciencies shown by letters (a & b)