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Development of transgenic B. juncea with ‘ech42’ gene (a) Cotyledon with petiole cultured on shoot regeneration medium (b) Explants showing regeneration on selection medium after infection with Agrobacteria. (c) Multiple shoots growing on selection medium (d) Elongated shoot transferred to rooting medium (e) Shoot with well developed roots (f) Transgenic plants transferred to soil for hardening (g) Hardened plants in pots showing flowering and pod setting
