. 2016 Mar 5;35:847–856. doi: 10.1007/s10096-016-2607-y

Table 1.

Primers targeting the genomic difference regions

Primer	Sequence 5′- > 3′	Gene in AUS0085 (ST203)	Gene product	PCR product size
Efm_R1_up	AATCGATGACGTGGAAGAAGG	Ef_aus00245	cadmium_translocating P-type ATPase	411 bp
Efm_R1_dn	GACTAAAGCGCCAGGACAAC	Ef_aus00245	cadmium_translocating P-type ATPase	411 bp
Efm_R2_up	ATGTTGCCCAAAAGACGAACC	Ef_aus 01495	riboflavin biosynthesis protein RibD	153 bp
Efm_R2_dn	GGAACGGCTAAAACAAGAAGC	Ef_aus 01495	riboflavin biosynthesis protein RibD	153 bp
Efm_R3_up	GCGTGATTTCGGTAATTGGTG	Ef_aus 02036	putative phosphosugar isomerase/binding protein	316 bp
Efm_R3_dn	ATGGGAATAGACCAGGAGCA	Ef_aus 02036	putative phosphosugar isomerase/binding protein	316 bp
Efm_R5_up	CGTGCGTTCCTTTTTCTACC	Ef_aus 02504	bacteriocin-like protein EntT	368 bp
Efm_R5_dn	GGTTTAGATAGCCCACCAAG	Ef_aus 02504	bacteriocin-like protein EntT	368 bp
Efm_R6_up	CCCATGAATCCTGTTGGTTC	Ef_aus 02768	PTS system, lactose/cellobiose-specific IIC component	182 bp
Efm_R6_dn	GCAAAAGTAGCAGGAAGGAC	Ef_aus 02768	PTS system, lactose/cellobiose-specific IIC component	182 bp
Efm_R7_up	TCAGCAAATGATGGCGATACG	Ef_aus 02778	glycosyl hydrolase family 38 protein	374 bp
Efm_R7_dn	ACCAATTCGGAGGAATGACATC	Ef_aus 02778	glycosyl hydrolase family 38 protein	374 bp