Fig. 5.

Effect of anti-RANKL mAb on bone marrow gene expression. Gene expression was assessed in bone marrow at the RNA level. Pro-osteoclastic genes (C-fms, RANK, and RANKL), osteoclast genes (CTSK and Dcstamp), and apoptosis-related genes (Bcl2, Bak, Bad , Bax, and Bim) were analyzed. Results were normalized by GAPDH expression. C-fms (a), RANK (b), and RANKL (c) expression was significantly elevated in the mAb group. No differences were found in the expression of CTSK (d) and Dcstamp (e) between the groups. No differences were found in the expression of Bcl2 (f), Bak (g), and Bad (h) between the groups. The expression of Bax (i) and Bim (j) were significantly stimulated in the mAb group. n ≥ 7/ group; *p < 0.05, **p < 0.01