Figure 3.

BMD₁₀ analysis of in vitro MN dose responses in human lymphocytes albendazole (green) flubendazole (black), R-reduced flubendazole (dark blue), S-reduced flubendazole (light blue) and albendazole sulfoxide (red), in human lymphocytes. The top figure shows that the four parameter (m5-bv) exponential model provided a suitable fit to each dose response using the compound as covariate for BMD analysis. The dotted lines show the BMD₁₀ derivations for each dose response, and these correspond to the mid-point for each line plotted in the bottom figure. These lines in the bottom graph span the BMDL₁₀ to the BMDU₁₀ derived from the top figure using PROAST v50.8. Albendazole and flubendazole were equipotent, and the R- and S-metabolites of flubendazole were less potent and of similar potency to albendazole sulfoxide. Hydrolysed flubendazole showed no dose response using BMD analysis and was omitted from this covariate analysis.