FIGURE 4.

Adoptive transfer of Tregs alters hepatic Th17/Treg balance and serum IL-10 levels. The mice were administered with CD4⁺CD25⁺Tregs or CD4⁺CD25^-effector T cells (1 × 10⁶; i.v.) 24 h before TP administration, then underwent TP administration (500 μg/kg; i.g.) and sacrificed 24 h after TP administration. Relative expressions of FoxP3 (A), ROR-γt (B), and IL-10 (C) were measured by real-time RT-PCR and normalized to GAPDH mRNA. The serum IL-10 level was measured by ELISA (D). The data are shown as the mean ± SEM of 6 mice. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. control. ^#P < 0.05 and ^###P < 0.001 vs. TP group.