Fig. 8.

Biochemical evidence of myofilament protein phosphorylation following sustained (14-day) β-AR stimulation and subsequent acute β-AR stimulation

Effects of acute i.p. DOB injection (DOB) in saline-infused (Control) and ISO-infused (ISO) wild-type mice on the relative the abundance of non-phosphorylated (0P), mono-phosphorylated (1P) and bis-phosphorylated (2P) cTnI relative to the sum of bis-, mono- and un-phosphorylated moieties. (A) Representative PhosTag phosphate affinity SDS-PAGE and standard SDS-PAGE immunoblots. (B) Quantitative data on the abundance of bis-phosphorylated (2P) cTnI, as a percentage of total cTnI (sum of the signals from all 3 cTnI phospho-moieties). Vehicle-injected mice served as control. Animal numbers per treatment n = 4; values are shown as mean ± SE, *p < 0.05 vs. vehicle-injected Control, two-way-ANOVA with Tukey's post-test.