FIGURE 5.

Effect of α3 or α5 integrin silencing on SFK activation in A549 cells during interaction with H. capsulatum. A549 cells were transfected with α3 or α5 integrin-directed siRNA or with Negative Control (NC) siRNA for 24 h, and then, incubated with H. capsulatum yeasts for 3 h. After incubation with fungi, A549 cells were harvested, lysed, and then P-SFK (Tyr⁴¹⁶) was analyzed by Western blot. SFK was used as protein loading control. Relative SFK phosphorylation was determined by densitometric analysis of the bands obtained by Western blot, and values represent the ratio of the intensity of P-SFK band divided by the corresponding intensity of SFK band.