FIGURE 7.

Effect of membrane raft disruption on the localization of SFKs, α3 and α5 integrins in A549 cell DRMs during interaction with H. capsulatum. After incubation with H. capsulatum yeasts for 3 h, A549 cells were harvested, lysed and membrane cholesterol of cell homogenate was removed by methyl-β-cyclodextrin (+ MβCD). Control in the absence of MβCD (- MβCD) was also performed. Then, A549 cell DRMs were isolated by using a sucrose gradient and ultracentrifugation. Aliquots of DRM fractions (4–6) were submitted to SDS-PAGE. α3 and α5 integrins, P-SFK (Tyr⁴¹⁶) and SFK were analyzed by Western blot. Blots are representative of two independent experiments.