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. 2016 Apr 22;7:580. doi: 10.3389/fmicb.2016.00580

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Copyright © 2016 Maza and Suzuki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of filipin on IL-6 and IL-8 secretion by A549 cells during interaction with H. capsulatum. A549 cells were incubated in the absence or presence of 1 μg/ml filipin (FIL, a cholesterol-binding compound that disrupts membrane rafts). After 2 h, H. capsulatum yeasts (Hc) were added to these cultures. After 16 h, culture supernatants were collected, and IL-6 (A) and IL-8 (B) levels were determined by ELISA. To analyze basal cytokine levels, A549 cells were incubated in the absence of filipin and H. capsulatum (FIL -/Hc -).Values represent the mean of triplicate experiments ± the standard deviation. ^∗p < 0.01 when compared to A549 cells incubated in the absence of filipin and H. capsulatum (FIL -/Hc -). #p < 0.01 when compared to A549 cells incubated with H. capsulatum, in the absence of filipin (FIL -/Hc +). Similar results were obtained from three independent experiments.