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. 2016 Apr 20;18(4):213–222. doi: 10.1016/j.neo.2016.01.006

Figure 2.

Figure 2

MI-219 radiosensitizes prostate cancer cells in a p53-dependent manner. (A) Western blot analysis of LNCaP cells after treatment with 10 μM MI-219 and/or 10 Gy of radiation. (B) Clonogenic survival assays of LNCaP cells after treatment with either 10 μM MI-219 or 10 μM nutlin-3 and radiation (0, 2, 4, and 6 Gy). (C) Apoptosis (sub-G1 population) was analyzed in LNCaP cells after treatment with 10 μM MI-219, radiation (10 Gy), or both via FACS analysis. (D–E) Similar experiments were performed with LNCaP p53 knockdown cells. (F–P) Western blot analyses, clonogenic cell survival assays, and quantification of apoptotic cells were carried out in a similar fashion in 22RV1, PC3, and DU145 cells.