Figure 4.

CTCF-binding motifs showing the M1/core that specifically engages fingers 4–8 and the M2/upstream sequence that engages fingers 7–11, with overlapping binding of the middle fingers to M1 and M2 (Nakahashi et al. 2013). Fingers not engaged in sequence-specific contacts may nonetheless contribute to overall binding stability through nonspecific interactions. Note that the sequence as shown binds CTCF with the N terminus facing downstream (Renda et al. 2007; Nakahashi et al. 2013). The DNA-binding modules described by Rhee and Pugh (2011) based on a ChIP-exo study are highlighted as colored bars at the bottom of the motif.