Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 22;6:24934. doi: 10.1038/srep24934

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) HeLa cells were transfected with non-targeting siRNA (NT-Qiagen) or SGTA siRNA (from Sigma, Ambion, and Qiagen) and 4 h later were transfected with WT or Vpu-defective (delVpu) pNL4-3 HIV-1 molecular clones. siRNA transfection was repeated again the next day. One day later, cell and viral lysates were prepared and subjected to western blot analysis with HIV-Ig to detect the Gag precursor Pr55Gag (p55) and the CA protein p24, or antibodies specific for SGTA, tetherin, Vpu, or tubulin. (B) virus release efficiency (VRE) was calculated as the amount of virion-associated p24 (CA) relative to total Gag in cell and virus. VRE was set to 100% for NT siRNA. (C) relative expression of endogenous SGTA, NT siRNA was set to 100%. Data shown are ± SD from three independent experiments.