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. 2016 Apr 22;6:24934. doi: 10.1038/srep24934

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HeLa (A) and 293T (B) cells were transfected with WT or Vpu-defective (delVpu) pNL4-3 HIV-1 molecular clones with or without FLAG-tagged SGTA expression vector. One day posttransfection, cell and viral lysates were prepared and subjected to western blot analysis with HIV-Ig to detect the Gag precursor Pr55Gag (p55) and the CA protein p24, or anti- FLAG to detect FLAG-tagged SGTA, or anti-tetherin, or anti-Vpu antisera. (C,D) virus release efficiency (VRE) was calculated as in Fig. 1B, and VRE was set to 100% for no SGTA controls. (E,F) the ratio of p24 to p55 in cell lysates was calculated and set to 100% for no SGTA controls. Data shown are ± SD from three (D,F) or four (C,E) independent experiments. P values (two-tailed paired t-test): *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0005.