Skip to main content
. 2016 Apr 22;6:24934. doi: 10.1038/srep24934

Figure 5. The non-glycosylated tetherin species co-immunoprecipitates with SGTA in the presence of WT Vpu.

Figure 5

293T cells were transfected with the vector expressing FLAG-tagged SGTA without or with Vpu (WT or RKR mutant) and HA-tagged tetherin expression vectors. Twenty-four h post-transfection, cells were lysed and immunoblotted with anti-FLAG (top left panel), anti-Vpu (middle left panel), or anti-HA antibodies (lower left panel) or immunoprecipitated with agarose beads conjugated with anti-FLAG antibodies followed by immunoblotting with anti-FLAG (top right panel), anti-Vpu (middle right panel) or anti-HA antibodies (lower right panel) to detect FLAG-tagged SGTA, Vpu and HA-tagged tetherin, respectively. The mobility of molecular mass standards is shown on the right.