Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 22;6:24934. doi: 10.1038/srep24934

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) 293T cells were transfected with the vector expressing HA-tagged tetherin with or without Vpu and FLAG-tagged SGTA expression vectors. 24 h post-transfection, cells were labeled with [³⁵S]Met/Cys for 30 min and then chased for 0, 0.5, 1, 2, or 4 h. Cells were lysed and immunoprecipitated with anti-HA antibodies and analyzed by SDS-PAGE followed by fluorography. Positions of 26-kDa (glycosylated) and 23-kDa (non-glycosylated) tetherin are indicated. (B) the ratio of 23- to 26-kDa tetherin species was calculated from three independent experiments, ± SD. This ratio increased significantly over time in the presence of SGTA and Vpu, p < 0.01.