Figure 1. Characterisation of exosomes from in vitro cell culture.
(A) Representative TEM image of DU145 exosomes at 7900 magnifications. White arrows depict exosomes of ~100 μm with kidney bean like appearances. (B) Representative particle tracking of a DU145 exosomes using the NanoSight Instrumentation. (C) Detection of exosome surface markers, CD9, CD63, TSG101 and AGO2 in DU145 and PNT2 cells. Absorbance was measured by the release of europium using a modified ELISA36. (D) Small RNA bioanalyser chip image showing the presences of small ncRNAs between 21 and 27 nts (black arrows).