Figure 3.

NAD⁺ protects primary neurons from TPrP-induced degeneration. (A) Primary cortical neurons were treated at 6 days in vitro with FK866 and TPrP as indicated, and rescued with NAD⁺. Cell viability was measured after 6 days of treatment. Statistical differences are shown compared to the matched controls as indicated in the graph. The experiment was done twice in duplicate wells; standard deviations are shown. (B) Where indicated, primary neurons were treated with 10 nM FK866, TPrP 10 µg/ml and NAD⁺ (various doses) for 6 days. Phase contrast microscopy pictures were taken at Day 6 except for FK866 alone where the picture was taken at Day 5. Pictures are representative of two replicates. **P < 0.01; ***P < 0.001.