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. Author manuscript; available in PMC: 2016 Apr 22.
Published in final edited form as: Free Radic Biol Med. 2015 Oct 9;89:1085–1096. doi: 10.1016/j.freeradbiomed.2015.09.024

Figure 2. Low expression of GLUT3 in HD mice and HD cells.

Figure 2

(a) qPCR analyses for GLUT3 from mRNA extracts of striatum from presymptomatic (3–4 weeks old) and symptomatic (8–12 weeks old) R6/2 mice. Striatum samples from littermates (WT) were used as controls. Results were normalised using specific primers to amplify mRNAs coding for β-actin. Analysis of variance (ANOVA) followed by the Bonferroni post-test,, n=4, ** p<0.01 and * p<0.05. (b) qPCR analyses for mRNA coding for GLUT3 in Q7 and Q111 cells. Student’s t-test, n=4, ** p<0.01. (c) Western blot assay for GLUT3 of total protein extracts from Q7 and Q111 cells. Bar plots show quantification of GLUT3 Western blot by densitometric scanning analysis, n=3, *** p<0.001, ** p<0.01. (d) Immunofluorescence analyses for GLUT3 (green) in Q7 and Q111 cells. Representative images for four independent experiments. Scale bar is 10 µm.