Figure 4.
Analysis of the epithelial-mesenchymal transition in vivo. Reverse transcription-quantitative polymerase chain reaction was used to analyze the mRNA expression levels of (A) E-cadherin and α-SMA, and (B) FN in the control, TGF-β1, and TGF-β1 treated with AM (10, 20 or 40 µg/ml) groups. Relative mRNA expression levels were quantified by densitometric analysis. Data are presented as the mean ± standard deviation. *P<0.05 vs. the control group; #P<0.05 vs. the TGF-β1 group. SMA, smooth muscle actin; FN, fibronectin; TGF, tumour growth factor; AM, Astragalus membranaceus.