Agarose gel electrophoresis result of eNOS G894T polymorphism provided by PCR-RFLP. The PCR product was digested with BanII restriction enzyme and visualized on a 2.5% agarose gel. Lanes 1, 2, 4, 5, 9, 10 show wild type GG genotype (163 and 85 bp); lanes 3,7,8 show heterozygous GT (248, 163 and 85 bp); and lane 6: homozygous TT (248 bp). Lane M: DNA marker. eNOS, endothelial nitric oxide synthase; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism.