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Akti-2 inhibits the sperm capacitation-associated tyrosine phosphorylation. Sperm (2×106/ml) were incubated in HMB for different times with or without Akti-2 at 37°C in 5% CO2, respectively. Protein tyrosine phosphorylation was assessed by western blot analysis. α-tubulin was used as the loading control. DMSO, dimethyl sulfoxide.
