Figure 4.

Akti-2 inhibits the sperm acrosome reaction. Detection of inhibition of Akti-2 on the sperm acrosome reaction. Akti-2 inhibited the sperm acrosome reaction induced by A23187 when co-incubated in HMB buffer for 30 min at 37≥C in 5% CO₂, by the (A) acid phosphatase activity assay ^(**P<0.01) and (B) Coomassie Brilliant Blue staining ^(**P<0.01). (C) The acrosome reaction induced by A23187 was reduced with Akti-2 (C1 and C3), compared with A23187 only (C2 and C4). The white arrows indicate the intact acrosome, and the black arrows indicate acrosome reacted.