Expression of miR141 in ESCC tissues. RT-qPCR was performed to investigate the expression of miR141 in ESCC tissues. The experiments were repeated ≥3 times. Bar graphs show the expression of miR141 in (A) ESCC and adjacent normal tissues (para-cancer), as well as in (B) ESCC tissues from patients with and without lymph node metastasis. Data are presented as the mean ± standard deviation. Error bars show standard error of the mean. (A) *P<0.05 vs. adjacent normal tissue; (B) *P<0.05 vs. the lymph node metastasis-free group. ESCC, esophageal squamous cell carcinoma; miR141, microRNA-141; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N1, with lymph node metastasis; N0, without lymph node metastasis.