Injection of β‐estradiol (E2) and GPER‐specific agonist G1 reduced serum IL‐1β and TNF‐α concentrations. (A) serum concentration of IL‐1β in 3, 6, and 24 h in postischemic rats in the control group (DMSO, black circle), β‐estradiol injected group (Red circle), G1‐injected group (Purple circle), and β‐Estradiol + G15injected group (Green circle); There is significant difference between E2 or G1 injection group with control group (E2, 24 h, P < 0.0001; G1, 3 h, P = 0.0036, 6 h, P = 0.0002, 24 h, P < 0.0001). There is no significant difference between E2 + G15 group with the control group (3 h, P = 0.8048; 6 h, P = 0.6572, 24 h, P = 0.1682). (B) serum concentration of TNF‐α postischemic rats in the control group (saline, black circle), β‐estradiol injection group (Red circle), and G1 injection group (Purple circle). There is significant difference between E2 or G1 injection group with control group (E2, 6 h, 24 h, P < 0.0001; G1, 3 h, P = 0.0420, 6 h & 24 h, P < 0.0001). There is no significant difference between E2 + G15 group with the control group (3 h, P = 0.9456, 6 h, P = 0.5393, 24 h, P = 0.2636). Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.