Table II.
Antibodies and conditions used for immunohistochemistry.
| Antibody | Catalogue no. | Clone | Species | Dilution/incubation | Pretreatmenta |
|---|---|---|---|---|---|
| Bcl-2 | M0887b | 124 | Mouse | 1:100/30 min | WB/pH 9 |
| CD34 | IR632b | QB END10 | Mouse | Ready to use/30 min | WB/pH 9 |
| CD44 | NCL-CD44–2c | DF 1485 | Mouse | 1:100/30 min | WB/pH 9 |
| CD117 (C-kit) | A4502b | C-kit | Rabbit | 1:200/30 min | WB/pH 9 |
| Desmin | IR606b | D33 | Mouse | Ready to use/30 min | WB/pH 9 |
| DOG-1 | NCL-L-DOG-1c | K9 | Mouse | 1:100/30 min | WB/pH 9 |
| Ki67 | M7240b | MIB-1 | Mouse | 1:500/30 min | WB/pH 9 |
| P16 | 725 4713d | EGH4 | Mouse | Ready to use/30 min | pH 9 |
| P53 | M7001b | DO7 | Mouse | 1:200/30 min | WB/pH 9 |
| PDGFRα | RB-9027-P1e | Not reported | Rabbit | 1:50/30 min | pH 9 |
| PHH3 | 369A-18f | Not reported | Rabbit | Ready to use/30 min | WB/pH 9 |
| S-100 | Z 0311b | Not reported | Rabbit | 1:2000/30 min | WB/pH 6 |
| SMA | M0851b | 1A4 | Mouse | 1:600/30 min | WB/pH 9 |
a
pH 6 or 9, heat-induced epitope retrieval in pH 6 or 9 antigen retrieval buffer (Dako,Glostrup, Denmark) and WB in a Pascal pressurized heating chamber (Dako). Purchased from
b
Dako
c
Novocastra/Leica Microsystems (Vienna, Austria)
d
Ventana (Tucson, AZ, USA)
e
Thermo Scientific (Waltham, MA, USA) and
f
Cell Morque (Rocklin, CA, USA). Bcl-2, B-cell lymphoma 2; DOG-1, discovered on GIST 1; PDGFRα, platelet-derived growth factor receptor α; PHH3, phosphohistone H3; SMA, smooth muscle actin; WB, waterbath; GIST, gastrointestinal stromal tumor; MIB-1, mindbomb E3 ubiquitin protein ligase 1.