Table 2.
Antibodies used in this study
| Antibody | Marker | Type | Origin (Reference) | Technique and Dilution |
|---|---|---|---|---|
| Anti-Alpha-Tubulin | Loading Control | Mouse Monoclonal | Sigma (T9026) | WB (1:1000) |
| Anti-BrdU | Transcription Assay | Mouse Monoclonal | Sigma (B8434) | IE (1:50) |
| Anti-Fibrillarin | Nucleolus | Mouse Monoclonal | ABCAM (ab4566) | IF (1:500) |
| Anti-Histone H2AX phospho-Ser139 | DNA Damage | Mouse Monoclonal | Millipore (05–636) | WB (1:1000) |
| IF (1:200) | ||||
| Anti-HP1 gamma | Heterochromatin | Mouse Monoclonal | Millipore (05–689) | IF (1:100) |
| Anti-RNA Pol II H5 antibody | Transcription sites | Mouse Monoclonal | Covance (MMS-129R) | IE (1:50) |
| Anti-TMG Cap | Nuclear Speckle (IGC) | Mouse Monoclonal | Oncogene (NA02A) | IF (1:100) |
| Anti-WRAP53 | DNA Damage | Mouse Monoclonal | ABNOVA (H00055135-M04) | WB (1:2000) |
| IF (1:200) | ||||
| IE (1:100) | ||||
| Anti-53BP1 | DNA Damage/repair | Rabbit Polyclonal | Bethyl Laboratories (A300-272A) | WB (1:1000) |
| IF (1:250) | ||||
| IE (1:50) | ||||
| Anti-ATM Phospho-Ser1981 | DNA Damage | Rabbit Polyclonal | Cell Signaling (4526) | WB (1:500) |
| IF (1:100) | ||||
| Anti-Coilin 210.4 antibody | Cajal Body | Rabbit Polyclonal | Provided by Prof. A.I. Lamond | IF (1:250) |
| IE (1:50) | ||||
| Anti-Fibrillarin 12.3 antibody | Nucleolus | Rabbit Polyclonal | Provided by Prof. M.Carmo-Fonseca | IE (1:100) |
| Anti-Histone H2AX phospho-Ser139 | DNA Damage | Rabbit Polyclonal | Novus (NB100-384) | IF (1:200) |
| Anti-Trimethyl-Histone H4 (Lys 20) | Heterochromatin | Rabbit Polyclonal | Millipore (07–463) | IF (1:250) |
| Anti-Ubiquityl H2A | DNA Damage | Rabbit Polyclonal | Millipore (05–678) | WB (1:1000) |
| IF (1:100) |
WB western blotting, IF immunofluorescence microscopy, IE immunogold electron microscopy