Fig. 2.

Testing the lingcod PCR assay. The PCR assay strategy is shown in a. The external primers (green polygons) bind both versions while the internal primer binds only in the male due to three internal mismatches. Mismatches are coloured lines, image produced in Geneious v8.0.4 [12]. In b, amplification of four male and four female lingcod templates using the Oelo-Sex three primer PCR assay. Presence of the 129 bp band denotes the presence of the male-specific copy of amh, while the 420 bp band is the shared autosomal copy. Ladder used is 1 kb O’generuler plus (Thermo), 2.0 % agarose gel stained with ethidium bromide