Figure 3.

Inhibition of OGT using RNAi-OGT decreased the colony formation and proliferation ability of the Eca-109 cells. Colony formation assay of the (A) control and (B) RNAi-OGT groups. (C) Colony formation ratio between the control and RNAi-OGT groups was calculated from the amount of colonies in 3 regions of 1 cm² in size on each plate, which was compared between the two groups used Student's t test (P=0.339). (D) Cellular proliferation curves (P=0.317). RNAi, RNA interference; OGT, O-linked N-acetylglucosamine transferase; OD, optical density.