Table I.
Sequence | ||
---|---|---|
shRNA segment | Top strand | Bottom strand |
RNAi-OGTA | 5′-TGCTGGATGTGCCAACTCAGC | 5′-CCTGGATGTGCCAACAGCTAA |
TAACCGTTTTGGCCACTGACTG | CCGTCAGTCAGTGGCCAAAACG | |
ACGGTTAGCTGTTGGCACATC-3′ | GTTAGCTGAGTTGGCACATCC-3′ | |
RNAi-OGTB | 5′-TGCTGTAGAGTAGGCATCAGC | 5′-CCTGTAGAGTAGGCAAGCAA |
AAAGGGTTTTGGCCACTGACTG | AGGGTCAGTCAGTGGCCAAAAC | |
ACCCTTTGCTTGCCTACTCTA-3′ | CCTTTGCTGATGCCTACTCTAC-3′ | |
RNAi-OGTC | 5′-TGCTGAATACTGCTCAGCAAC | 5′-CCTGAATACTGCTCAAACTTC |
TTCAGGTTTTGGCCACTGACTG | AGGTCAGTCAGTGGCCAAAACC | |
ACCTGAAGTTTGAGCAGTATT-3′ | TGAAGTTGCTGAGCAGTATTC-3′ | |
Negative control | 5′-GAAATGTACTGCGCGTGGAG | |
ACGTTTTGGCCACTGACTGACG | ||
TCTCCACGCAGTACATTT-3′ |
shRNA, short hairpin RNA; RNAi, RNA interference; OGT, O-linked N-acetylglucosamine transferase.