Figure 2.

(A) Array comparative genomic hybridization confirmed the deletion in TP53, which was detected initially using iFISH and multiplex ligation dependent probe amplification for CLL cases C-21 and C-22. The whole short arm was deleted and the long arm was possibly duplicated due to an isochromosome 17 formation, at least in case C-21. (B) Examples for gain of copy numbers for BIRC3 and MALT1 in the 2 cases by iFISH: i) C-21, an example of 3 copies and ii) C-22, an example of 4 copies. (C) iFISH results of the CLL case C-23. BIRC3 had 3 copies in certain cells; however ATM, MALT1 and TP53 exhibited only 2 copies each, in all cells. TP53, tumor protein 53; CLL, chronic lymphocytic leukemia; iFISH, interphase fluorescence in situ hybridization; BIRC3, baculoviral IAP repeat-containing 3; MALT1, mucosa-associated lymphoid tissue lymphoma translocation gene 1; ATM, ataxia telangiectasia mutated.