FIG. 3.

Cytotoxicity of three types of TIs as evaluated by the culturing of MC3T3-E1 cells on the TIs for 2 days. (A) qRT-PCR analyses for expressions of a proapoptotic gene (Bax) and an antiapoptotic gene (Bcl-2). The expression levels were normalized to those of the glass group (n = 4). No significant difference was observed among the three different types of TIs. (B) Apoptotic activity of MC3T3-E1 cells on the TIs as evaluated by immunocytochemistry for caspase-3 (red). Blue indicates nucleus (DAPI). Scale bars indicate 100 μm. No significant difference was observed among the three different types of TIs. qRT-PCR, quantified reverse transcription-polymerase chain reaction. Color images available online at www.liebertpub.com/tea