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. Author manuscript; available in PMC: 2016 Apr 22.
Published in final edited form as: Curr Biol. 2014 Dec 31;25(2):163–174. doi: 10.1016/j.cub.2014.11.040

Figure 6. Optogenetic Stimulation of ADF and NSM Neurons Increases Thermotolerance and Suppresses polyQ Aggregation in Body Wall Muscle Cells.

Figure 6

(A) Experimental set up (as described in Figure 5 legend).

(B) Thermotolerance of experimental (+ATR) versus control (−ATR) animals following optogenetic stimulation. n = 80–83 animals per condition; two-way ANOVA; *p < 0.05.

(C) Q35::YFP aggregates visualized in head-muscle cells of control (−ATR) and experimental (+ATR) animals 3 days following optogenetic stimulation.

(D) The number of Q35::YFP aggregates in control (−ATR) and experimental (+ATR) animals over time. n = 7–10 animals per time point per experimental manipulation, t test; ***p < 0.001.

(E) The number of Q35::YFP aggregates in control (−ATR) and experimental (+ATR) animals in the absence of optogenetic stimulation.

(F) The number of Q35::YFP aggregates following RNAi-mediated knockdown of HSF1 in experimental (+ATR) animals following optogenetic stimulation. n = 26, t test; **p < 0.01.