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. 2016 Mar 3;27(4):309–324. doi: 10.1089/hum.2016.008

Figure 5.

Figure 5.

Disruption of MT-MTOC increases viral transduction and nuclear entry through an RhoA-ROCK-Actin pathway. (A) Immunoblot showing the siRNA-mediated transient knockdown of the RhoA protein in Hela cells. Scramble siRNA used as a control. (B) The level of rAAV transduction was measured in Scramble or RhoA siRNA-transfected cells treated with or without nocodazole treatment at 6–8 hr after rAAV infection. (C) The level of rAAV transduction was determined in cells treated with DMSO or nocodazole alone or in combination with H1152 or CytoD. (D) The proportion of cells with perinuclear accumulation of rAAV particles was measured in the presence of DMSO or nocodazole alone or in combination with H1152 or CytoD. (E) The proportion of rAAV particles in the nucleus was determined in cells treated with DMSO or nocodazole alone or in combination with H1152 or CytoD. *Statistically significant difference between DMSO only and other drug treatments with p < 0.01.