Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 16;11(5):3054–3060. doi: 10.3892/ol.2016.4338

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 2. — siRNA knockdown of TLR4 expression in the human liver cancer HepG2 cell line. (A) Results of reverse transcription-polymerase chain reaction of RNA extracted from HepG2 cells transfected with siRNA-1, −2 or −3, the blank control, negative siRNA or Lipo. β-actin mRNA was amplified as a control. (B) Levels of TLR4 mRNA expression in HepG2 cells transfected with siRNA-1, −2 or −3 and the three control groups. The data are expressed as the mean ± SD of TLR4 mRNA levels for each group. *P<0.05 vs. control groups (blank control, negative siRNA-transfected and Lipo-transfected cells). (C) Western blotting of protein extracted from siRNA-1-transfected, blank control, negative siRNA-transfected and Lipo-transfected HepG2 cells. β-actin was included as a loading control. (D) Levels of TLR4 protein expression in HepG2 cells transfected with siRNA-1, −2 or −3 and the three control cells, assessed using western blotting. The data are expressed as the mean ± SD of TLR4 protein levels for each group. *P<0.05 vs. control groups (blank control, negative siRNA-transfected and Lipo-transfected cells). Students t-test and analysis of variance were used to determine the significance of differences between groups. siRNA, small interfering RNA; TLR4, Toll-like receptor 4; Lipo, Lipofectamine 2000; mRNA, messenger RNA; SD, standard deviation.