Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 21;8:44. doi: 10.1186/s13073-016-0293-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Grassl et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Workflow for ultra-deep and quantitative saliva proteomics. (1, 2) Saliva is collected with a sterile cotton swab and its proteins are denatured, digested, and purified according to the iST protocol [12]. (3) Depending on the desired proteome depth, samples are either separated into eight fractions or directly measured in single runs. (4) Peptides are measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS). (5) MaxQuant identifies and quantifies the proteins and enables statistical analysis in the Perseus software environment. The required time for each of the steps is indicated below each panel