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. 2016 Mar 16;11(5):3197–3201. doi: 10.3892/ol.2016.4339

Figure 3.

Figure 3.

Overexpression of AMPK renders renal cancer cells resistance to radiation. (A) DLD-1 cells were transfected with AMPK or control vector for 48 h, the expression of total AMPK and phospho-AMPK at T172 were measured by western blot analysis. GAPDH was a loading control. (B) DLD-1 cells were transfected with AMPK or control vector for 48 h, followed by irradiation at 0, 0.5, 1, 2, 4 and 8 Gy. Then cells were analyzed by cell viability assay. (C) DLD-1 cells were treated with Metformin at the indicated concentrations for 1 h, then the phospho-AMPK at T172 were measured by western blot, the relative intensities were showed as folds increase. (D) DLD-1 cells were treated with Metformin at the indicated concentrations for 1 h, then cells were exposed to radiation at 0, 1, 2, 4, 8 and 16 Gy, followed by the measurements of cell viability. Columns, mean of three independent experiments; bars, standard error. *P<0.05 vs. control group; **P<0.01 vs. control group; ***P<0.001 vs. control group. AMPK, adenosine monophosphate-activated kinase; t-AMPK, total AMPK; p-AMPK, phospho-AMPK; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; T172, phosphorylation site on Threonine 172.