FIG. 4.

QGel 3D Hydrogel proliferation and adipogenic differentiation. (A) Proliferation of the ASCs in maintenance conditions in 3D culture as determined by a CyQUANT assay. (B) 3D reconstructions of ASCs adipogenically differentiated for 21 days in Qgel containing various attachment peptides. (C) The average number of nuclei visualized per field showing the overall number of cells present after 21 days of adipogenic culture. (D) The percent of total cells present in each field that are associated with a lipid vacuole. (E) The average size of all the lipid vacuoles present for each condition (*p < 0.05, **p < 0.01, ***p < 0.001). 3D, three-dimensional.