Table 1. Hydrodynamic dimensions of recombinant bOBPwt and its mutant forms in the absence and in the presence of natural ligand OCT in different structural states.

	GdnHCl, M	First peak, kDa	Second peak, kDa
bOBPwt	0.0	43.9	23.8
	0.5	34.0	19.3
	1.5	43.6
bOBPwt/OCT	0.0	39.6	21.5
	0.55	27.2	17.0
	1.7	39.6
bOBP/Gly121+	0.0	23.6
	0.25	17.8
	1.5–1.9	24.8–28.5
bOBP/Gly121+/OCT	0.0	21.5
	0.24–0.5	15.5–16.2
	1.7–2.0	24.7–28.5
GCC-bOBP	0.0	23.6
	0.25	17.8
	1.1–1.82	22.5–25.9
GCC-bOBP/OCT	0.0	22.5
	0.27	16.9
	1.5–2.0	22.5–27.2