Table 2. Thermodynamic parameters of GdnHCl-induced denaturation of bOBP, its mutant variants and their complexes with octen-3-ol (OCT).

Protein	m (kJmol−1M−1)	Cm (M)a	$\Delta G_{N-U}^0$b (kJmol−1)
bOBP	3.7 ± 0.2	2.1 ± 0.1	7.7 ± 0.6
bOBP/OCT	4.1 ± 0.2	2.7 ± 0.1	11.0 ± 0.5
bOBP/Gly121+	2.8 ± 0.2	2.0 ± 0.1	5.5 ± 0.4
bOBP/Gly121+/OCT	5.3 ± 0.2	2.6 ± 0.1	14.0 ± 0.5
GCC-bOBP	2.5 ± 0.3	2.3 ± 0.1	5.8 ± 0.7
GCC-bOBP/OCT	4.5 ± 0.3	2.7 ± 0.1	12.0 ± 0.9

Notes.

^aC_m is the denaturant concentration at midpoint of conformational transition.

^bThe fluorescence signals of the folded and unfolded states were approximated by linear dependences as function of denaturant concentration (Nolting, 1999).