Table 5. Comparison of fold change differences of 29 transcripts between EO and muscle measured by qRT-PCR and RNA-seq.

qRT-PCR measurements were normalized against the geometric means of two internal reference genes: rps11, and snrpb. For all qRT-PCR ratios, n = 4. Statistical analysis of the qRT-PCR dataset identified five significantly differentially expressed transcripts (p ≤ 0.05, designated ‘*’). All RNA-seq ratios (n = 1) were considered to be differentially expressed if the difference in transcript expression was more than 4-fold different between EO and muscle. †—To date, we have not resolved the identity of all fast-twitch and slow-twitch myosin heavy chain isoforms transcribed in muscle and EO of S. macrurus and therefore we did not include RNAseq data for myh-f and myh-s in this table.

(A) Sarcomeric structure
		hsp70	hsp90a2	plec	ank2	dmd	cryaba	actn2	myoz3	myom1	ckma	ttnB	myh10	mylk4	myh-f	myh-s
qRT-PCR	Fold-change	2.37	0.41	2.28	5.89*	1.26	1.03	0.77	0.72	0.35	0.38	0.66	3.88	0.90	0.22	0.02
	Standard error	0.16	0.05	0.10	0.34	0.02	0.12	0.14	0.13	0.01	0.02	0.05	0.57	0.07	0.03	0.01
	P-value	0.08	0.18	0.10	0.04	0.38	0.99	0.65	0.38	0.08	0.14	0.66	0.08	0.99	0.26	0.09
	RNA-seq	Fold-change	3.54	0.70	3.61	4.75	2.24	6.46	0.20	1.50	0.62	0.70	0.70	12.19	0.33	†	†